The air ambulance was called out last night after a motorbike collided with a car on Edlington Lane, Edlington.
The incident happened near the Asda supermarket, and it is believed an off bike collided with a a car pulling out of a junction.
The road was closed for several hours as police investigated.
A spokesman for the Yorkshire Ambulance Service said: “We received a call at 4.28pm and sent a land ambulance, and the air ambulance was dispached. The air ambulance conveyed one patient, the rider of the motorbike, to the Northern General Hospital in Sheffield.”