Doncaster Mustangs Youth Academy will host a four-team tournament at the Keepmoat Stadium on Saturday.
The event, which features three Yorkshire sides, has been organised to spread the word about American Football in the county.
It will also serve as a rehearsal for the British American Football Association National League (BAFANL) junior BritBowl finals, which will be held at the Keepmoat’s athletics arena in September.
Doncaster Mustangs’ juniors, Sheffield Predators, Yorkshire Rams (Leeds) and Coventry Jets will compete in this weekend’s competition.
The opening round of games start at 1pm, the second round of matches at 2.15pm, and the final round of games at 3.30pm.
Organiser Steve Whitter said: “Hopefully this event will highlight American Football in Doncaster, South Yorkshire and in Yorkshire as a whole.”